미니돼지에서 다능성 피부유래 전구세포의 추출과 이의 다배엽 세포로의 분화유도에 대한 연구
ISOLATION OF PORCINE MULTIPOTENTIAL SKIN-DERIVED PRECURSOR CELLS AND ITS MULTILINEAGE DIFFERENTIATION
최문정, 변준호, 강은주, 노규진, 김종렬, 김욱규, 박봉욱,
소속 상세정보
최문정 ( Choi Mun-Jeoung ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
변준호 ( Byun June-Ho ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
강은주 ( Kang Eun-Ju ) - 경상대학교 수의과대학
노규진 ( Rho Gyu-Jin ) - 경상대학교 수의과대학 동물의학연구소
김종렬 ( Kim Jong-Ryoul ) - 부산대학교 치의학전문대학원 구강악안면외과학교실
김욱규 ( Kim Uk-Kyu ) - 부산대학교 치과대학 구강악안면외과학교실
박봉욱 ( Park Bong-Wook ) - 경상대학교 의학전문대학원 치과학교실 구강악안면외과
KMID : 0355620080340060588
Abstract
There are increasing reports regarding regeneration of the defected tissues using tissue engineering technique. In this technique, multipotential stem cells are essential. There are many potential sources of adult stem cells, such as bone marrow, umbilical cord blood, fat, muscle, dental tissues and skin. Among them, skin is highly accessible and easily obtained with a minimum of donor site complications. Moreover, skin is an abundant adult stem cell sources and has the potential for self-replication and immune privilege. In this study, we isolated skin-derived precursor cells (SKPs) from the ear of adult miniature pigs. In these SKPs, the expression of transcriptional factors, Oct-4, Sox-2, and Nanog were detected by RT-PCR. In vitro osteogenesis and adipogenesis were observed at 3 weeks after transdifferentiations as assayed by positive von Kossa and Oil-red O staining, respectively. In addition, expression of osteocalcin and osteonectin in the osteogenic differentiation medium and PPARγ2 and aP2 in the adipogenic differentiation medium were detected by RT-PCR. In vitro neurogenesis of porcine SKPs was observed during 24 and 72 hours after treatment of neurogenic differentiation medium. The results of this study suggest that SKPs demonstrate the properties of pluripotence or multipotence and multi-lineage differentiation. This indicates that autogenous SKPs are a reliable and useful source of adult stem cells for regenerative medicine.
키워드
Skin-derived precursor cells (SKPs);Tissue-engineering;Adult stem cell.
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